VitaTaq® HS PCR Kit

Procomcure — Mon, 22 Jul 2019 13:40:11 +0000

VitaTaq^® HS DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results. The included HotStart feature allows full control over the reaction start and protects the enzyme for maximum performance.

Applications

Routine PCR for fragments up to 5 kb
Colony PCR
High-throughput PCR
Realtime PCR
Cycle sequencing

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	Yes
Fidelity	1X Taq
GC-Rich PCR Performance	moderate
Product Overhang	3'-A
Reaction Speed	Standard (1 min / kb)
Product Size	200 units / 500 units / 2500 units
Endonuclease Activity	none detected
5’-3’ Exonuclease Activity	yes
3’-5’ Proofreading Activity	none detected
Incorporation of labeled nucleotides	yes
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

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VitaTaq® HS PCR Kit