PhoenixDx® RT-qPCR Kit – Performance

Kathrin Breunig — Tue, 08 Sep 2020 11:33:55 +0000

PhoenixDx® RT-qPCR Kit – Performance contains everything needed for a successful RT-PCR experiment. The 2X qPCR Mix contains dNTPs and an optimized buffer formulation to provide the right environment for successful reverse transcription and PCR amplification in one reaction. The 20X RT Enzyme Mix provides the enzymes for RT-PCR: VitaScript® Reverse Transcriptase for precise and sensitive reverse transcription of RNA to cDNA and VitaTaq® DNA Polymerase for fast and reliable amplification of your sequence of interest. Procomcure’s proprietary HotStart antibody grants full control over the reaction start and protects the enzymes under harsh reaction conditions.

PhoenixDx® RT-qPCR Kit – Performance is optimized for fast amplification and multiplex applications making it the best option when high throughput is required.The reverse transcription reaction uses a novel VitaScript® Reverse Transcriptase with increased performance, specificity and reduced RNAse H activity.

In 1Step-RT-PCR, reverse transcription and PCR amplification are combined in 1 reaction granting several advantages:

- - Minimal handling steps reduce the risk of errors
  - Experiment setup and running time are reduced
  - A high sample number can be screened easily

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PhoenixDx® RT-qPCR Kit – Performance