PhoenixDx Kit Clostridium difficile (ToxinB) is a detection system for Clostridium difficile DNA based on real-time PCR. The kit allows for rapid and sensitive detection of Clostridium difficile DNA previously purified from various samples.
PhoenixDx Kit Clostridium difficile (ToxinB) detects tcdB, a gene highly specific for members of Clostridium difficile. Presence of tcdB in the purified DNA sample is measured through a probe-specific FAM-signal at 530 nm.
The kit also contains a PCR positive control (PPC, measured through a Cy5-Signal at 667 nm). The PPC helps to exclude false-negative test results e.g. through flawed DNA extraction or incorrect reaction setup. For well-to-well normalization, ROX is included as a passive reference dye. This helps to compensate for pipetting inaccuracies and improves overall data quality. However, the kit can be used without ROX normalization as well. Before starting the experiment, please make sure that your device is equipped and calibrated for FAM, Cy5 (and ROX if required).