PhoenixDx Kit Legionella pneumophila is a detection system for L. pneumophila DNA based on real-time PCR. The kit allows for rapid and sensitive detection of L. pneumophila DNA previously purified from various samples.
PhoenixDx Kit Legionella pneumophila detects a part of the mip gene which is highly specific for L. pneumophila. Presence of this specific DNA sequence in the purified DNA sample is measured through a probe-specific FAM-signal at 530 nm.
The kit also contains a PCR positive control (PPC, measured through a Cy5-Signal at 667 nm). The PPC helps to exclude false-negative test results e.g. through flawed DNA extraction or incorrect reaction setup and can be used for a general evaluation of the experiment. For well-to-well normalization, ROX is included as a passive reference dye. This helps to compensate for pipetting inaccuracies and improves overall data quality. However, the kit can be used without ROX normalization as well. Before starting the experiment, please make sure that your device is equipped and calibrated for FAM, Cy5 (and ROX if required).