PhoenixDx Kit Listeria monocytogenes is a detection system for L. monocytogenes DNA based on real-time PCR. The kit allows for rapid and sensitive detection of L. monocytogenes DNA previously purified from various samples.
PhoenixDx Kit Listeria monocytogenes detects beta hemolysin A (hlyA), a virulence factor highly specific for L. monocytogenes. Presence of hlyA in the purified DNA sample is measured through a probe-specific FAM-signal at 530 nm.
The kit also contains a PCR positive control (PPC, measured through a Cy5-Signal at 667 nm). The PPC helps to exclude false-negative test results e.g. through flawed DNA extraction or incorrect reaction setup and can be used for a general evaluation of the experiment. For well-to-well normalization, ROX is included as a passive reference dye. This helps to compensate for pipetting inaccuracies and improves overall data quality. However, the kit can be used without ROX normalization as well. Before starting the experiment, please make sure that your device is equipped and calibrated for FAM, Cy5 (and ROX if required).