Mycoplasma are the most common contamination in cell culture. They can affect cell culture in many different ways depending on the cell line, cultivation conditions etc. making it difficult to pinpoint them as the source of a problem. In general, an infection with mycoplasma will at least interfere with proper data collection and precious time and money is lost.
Realtime-PCR can detect Mycoplasma contamination at an early stage. It is an easy, cost-effective and fast testing method that can easily integrated into laboratory routine and help to ensure high-quality data from clean experiments.