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VitaTaq^® DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results.

VitaTaq^® PCR Kit contains VitaTaq^® DNA Polymerase and an optimized 10X Reaction Buffer for maximum flexibility regarding the reaction setup.

Applications

• Routine PCR for fragments up to 5 kb
• Colony PCR
• High-throughput PCR
• Realtime PCR
• Cycle sequencing

HotStart Antibody is a highly pure antibody that binds to Taq DNA Polymerase and thereby blocks its polymerase activity. At the very beginning of the thermal cycling protocol, the antibody is inactivated and with this “Hot Start”, Taq DNA Polymerase regains its function. This highly controlled reaction start prevents non-specific amplification and grants maximum control over the reaction conditions.1–3

Features

• Inhibits polymerase activity until desired
• Enhances polymerase performance & stability under harsh cycling conditions
• No DNA contaminations detected with PCR

RNase inhibitors (ribonuclease inhibitors) are recombinant enzymes used to inhibit RNase activity during your experiments. RNase inhibitors are commonly used as a precautionary measure in enzymatic manipulations of RNA to inhibit and control for such contaminants. Small amounts of ribonucleases can co-purify with isolated RNA and compromise downstream applications. Such contamination can also be introduced via tips, tubes, and other reagents used in procedures.

Applications

• first-strand synthesis of cDNA from RNA molecules and RT-PCR

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)), is an RNAdependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). This is a form of M-MLV Reverse Transcriptase that has been genetically altered to remove the associated RNase H activity.

Applications

• first-strand synthesis of cDNA from RNA molecules and RT-PCR