HotStart | Procomcure Biotech https://shop.procomcure.com Quality without compromise Wed, 31 Jan 2024 16:16:58 +0000 de hourly 1 https://wordpress.org/?v=6.7.5 https://shop.procomcure.com/wp-content/uploads/2018/11/cropped-PCC-Logo-new-32x32.png HotStart | Procomcure Biotech https://shop.procomcure.com 32 32 HotStart Antibody https://shop.procomcure.com/produkt/hotstart-antibody/?utm_source=rss&utm_medium=rss&utm_campaign=hotstart-antibody&utm_source=rss&utm_medium=rss&utm_campaign=hotstart-antibody Tue, 23 Jul 2019 14:00:07 +0000 https://shop.procomcure.com/?post_type=product&p=1394 For convenience, 5 U/μl Taq polymerase can be mixed with an equal volume of HotStart antibody, aliquoted and stored at -20°C until further use.

The post HotStart Antibody first appeared on Procomcure Biotech.]]> HotStart Antibody is a highly pure antibody that binds to Taq DNA Polymerase and thereby blocks its polymerase activity. At the very beginning of the thermal cycling protocol, the antibody is inactivated and with this “Hot Start”, Taq DNA Polymerase regains its function. This highly controlled reaction start prevents non-specific amplification and grants maximum control over the reaction conditions.



Specifications
TargetTaq DNA Polymerase
ConjugationUnconjugated
Concentration5 U/µl
Storage-15°C to -25°C

Interested in bulk sizes?

Download the IFU here

The post HotStart Antibody first appeared on Procomcure Biotech.]]> VitaTaq® HS PCR Kit https://shop.procomcure.com/produkt/vitataq-hs-pcr-kit/?utm_source=rss&utm_medium=rss&utm_campaign=vitataq-hs-pcr-kit&utm_source=rss&utm_medium=rss&utm_campaign=vitataq-hs-pcr-kit Mon, 22 Jul 2019 13:40:11 +0000 https://shop.procomcure.com/?post_type=product&p=1389 VitaTaq® HS DNA Polymerase is a robust and reliable Taq DNA polymerase suitable for all common PCR applications like colony PCR, cloning applications, high-throughput PCR and routine PCR.

The post VitaTaq® HS PCR Kit first appeared on Procomcure Biotech.]]> VitaTaq® HS DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results. The included HotStart feature allows full control over the reaction start and protects the enzyme for maximum performance.

Applications

  • Routine PCR for fragments up to 5 kb
  • Colony PCR
  • High-throughput PCR
  • Realtime PCR
  • Cycle sequencing

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the MSDS here

Download the IFU here

Specifications

PolymeraseTaq DNA Polymerase
Hot StartYes
Fidelity1X Taq
GC-Rich PCR Performance moderate
Product Overhang 3'-A
Reaction SpeedStandard (1 min / kb)
Product Size200 units / 500 units / 2500 units
Endonuclease Activitynone detected
5’-3’ Exonuclease Activityyes
3’-5’ Proofreading Activitynone detected
Incorporation of labeled nucleotidesyes
Reaction Format Separate Components
Shipping Condition Approved for shipment on wet or dry ice
Storage-15°C to -25°C
The post VitaTaq® HS PCR Kit first appeared on Procomcure Biotech.]]>