qPCR | Procomcure Biotech

PhoenixDx® RT-qPCR Kit – Performance

Kathrin Breunig — Tue, 08 Sep 2020 11:33:55 +0000

PhoenixDx® RT-qPCR Kit – Performance contains everything needed for a successful RT-PCR experiment. The 2X qPCR Mix contains dNTPs and an optimized buffer formulation to provide the right environment for successful reverse transcription and PCR amplification in one reaction. The 20X RT Enzyme Mix provides the enzymes for RT-PCR: VitaScript® Reverse Transcriptase for precise and sensitive reverse transcription of RNA to cDNA and VitaTaq® DNA Polymerase for fast and reliable amplification of your sequence of interest. Procomcure’s proprietary HotStart antibody grants full control over the reaction start and protects the enzymes under harsh reaction conditions.

PhoenixDx® RT-qPCR Kit – Performance is optimized for fast amplification and multiplex applications making it the best option when high throughput is required.The reverse transcription reaction uses a novel VitaScript® Reverse Transcriptase with increased performance, specificity and reduced RNAse H activity.

In 1Step-RT-PCR, reverse transcription and PCR amplification are combined in 1 reaction granting several advantages:

- - Minimal handling steps reduce the risk of errors
  - Experiment setup and running time are reduced
  - A high sample number can be screened easily

The post PhoenixDx® RT-qPCR Kit – Performance first appeared on Procomcure Biotech.

PhoenixDx® RT-qPCR Kit – Robust

Kathrin Breunig — Tue, 08 Sep 2020 11:01:58 +0000

PhoenixDx® RT-qPCR Kit – Robust contains everything needed for a successful RT-PCR experiment. The 2X qPCR Mix contains dNTPs and an optimized buffer formulation to provide the right environment for successful reverse transcription and PCR amplification in one reaction. The 20X RT Enzyme Mix provides the enzymes for RT-PCR: VitaScript® Reverse Transcriptase for precise and sensitive reverse transcription of RNA to cDNA and VitaTaq® DNA Polymerase for fast and reliable amplification of your sequence of interest. Procomcure’s proprietary HotStart antibody grants full control over the reaction start and protects the enzymes under harsh reaction conditions.

PhoenixDx® RT-qPCR Kit – Robust is also highly resistant to a broad field of PCR inhibitors such as heparin (blood samples), serum or humic acid (soil / plant samples). This makes it a flexible solution compatible with many different sample types. RT-qPCR Kit – Robust can be used for many qPCR-based applications such as gene expression studies, RNA virus detection and many more.

Download the manual here
Download the MSDS here

The post PhoenixDx® RT-qPCR Kit – Robust first appeared on Procomcure Biotech.

PhoenixDx Mycoplasma Mix

Procomcure — Thu, 24 Jan 2019 14:44:02 +0000

Contamination with mycoplasma is amongst the most notorious issues associated with cell cultivation. Depending on cell type, source and culture methods, the contamination with mycoplasma, acholeplasma and ureaplasma varies between 15 and 80%1. A contamination is not only inconvenient, but also a costly issue as it often requires the elimination of precious cultures. Furthermore, the clinical use of cultured cells makes testing for mollicutes a necessity, especially for pathogens like M. pneumoniae, M. genitalium and M. hominis. PhoenixDx® offers a fast, sensitive and highly specific detection system for more than 130 mollicute species to provide certainty when certainty is needed.

qPCR for fast, sensitive and specific detection of mollicutes

Especially when working with virus samples in the cell culture, qPCR is superior to conventional detection methods as high concentrations of enveloped viruses can bias colorimetric methods like ATP conversion2. With PhoenixDx® Mycoplasma Mix, the 16s rDNA of more than 130 mollicute species is targeted covering Mycoplasma, Acholeplasma and even Ureaplasma, whereas genomic, eukaryotic DNA (e.g. from the cell culture) is not amplified. To exclude false-negatives due to PCR inhibition, an additional PCR positive control is included in the mastermix.
PhoenixDx® Mycoplasma Mix is not only highly specific, but also easy and fast to use due to its convenient 2X Mastermix formulation.

The PhoenixDx® Mycoplasma Mix includes:

• Primers for the amplification of the 16s rDNA region
• A PCR positive control
• FAM Probe for the detection of mollicutes
• HEX Probe for the detection of the PCR Positive Control
• dUTP for optional UNG treatment
• Taq-antibody and VitaTaq® Polymerase for High Performance and HOTSTART Control
• Optimized buffer system for an efficient lysis of intact mollicute cells
• Universal ROX concentration for maximum instrument compatibility

Download the IFU here

Read more in a recently published articel here,
or download the article here

The post PhoenixDx Mycoplasma Mix first appeared on Procomcure Biotech.

2X Magic Probe Mix

Procomcure — Fri, 16 Nov 2018 13:20:14 +0000

2X Magic PROBE Mix is a convenient 2X qPCR Mastermix for probe-based detection (such as TaqMan) with a universal concentration of ROX compatible with all common thermocycling devices (including Bio-Rad devices). A dTTP/dUTP blend allows for pre-PCR UNG digestion if cross-contamination is an issue. 2X Magic Probe Mix an easy-to-use, all-round talent for difficult qPCR applications as well as for regular applications.

Applications

Quantitative PCR
Realtime PCR

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	yes
Detection	Probe
Reaction Speed	Fast / Standard
Product Size	200 / 500 reactions à 20 µl
Normalization	universal ROX concentration
Sample type	DNA, cDNA
Reaction Format	2X Mastermix
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post 2X Magic Probe Mix first appeared on Procomcure Biotech.

2X Magic SYBR Mix

Procomcure — Fri, 16 Nov 2018 13:16:54 +0000

2X Magic SYBR Mix is a convenient 2X qPCR Mastermix for SYBR® Green-based detection with a universal concentration of ROX compatible with all common thermocycling devices (including Bio-Rad devices). A dTTP/dUTP blend allows for pre-PCR UNG digestion if cross-contamination is an issue. 2X Magic SYBR Mix an easy-to-use, all-round talent for difficult qPCR applications as well as for regular applications.

Applications

Quantitative PCR
Realtime PCR
Genexpression Analysis
High-throughput applications

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	yes
Detection	SYBR Green
Reaction Speed	Fast / Standard
Product Size	200 / 500 reactions à 20 µl
Normalization	universal ROX concentration
Sample type	DNA, cDNA
Reaction Format	2X Mastermix
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post 2X Magic SYBR Mix first appeared on Procomcure Biotech.

2X ProPlant Probe Mix

Procomcure — Fri, 16 Nov 2018 13:06:44 +0000

2X ProPlant Probe Mix is a convenient 2X qPCR Mastermix for probe-based detection (such as TaqMan) with a universal concentration of ROX compatible with all common thermocycling devices (including Bio-Rad devices). A dTTP/dUTP blend allows for pre-PCR UNG digestion if cross-contamination is an issue. 2X ProPlant Probe Mix an easy-to-use, all-round talent for difficult qPCR applications as well as for regular applications.
2X ProPlant Probe Mix shows increased resistance against plant derived PCR inhibitors.

Applications

Quantitative PCR
Realtime PCR
Analysis of plant derived samples

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	yes
Detection	Probe-based
Reaction Speed	Fast / Standard
Product Size	200 / 500 reactions à 20 µl
Normalization	universal ROX concentration
Sample type	DNA, cDNA
Reaction Format	2X Mastermix
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post 2X ProPlant Probe Mix first appeared on Procomcure Biotech.

2X ProPlant SYBR Mix

Procomcure — Fri, 16 Nov 2018 13:02:23 +0000

2X ProPlant SYBR Mix is a convenient 2X qPCR Mastermix for SYBR® Green-based detection with a universal concentration of ROX compatible with all common thermocycling devices (including Bio-Rad devices). A dTTP/dUTP blend allows for pre-PCR UNG digestion if cross-contamination is an issue. 2X ProPlant SYBR Mix an easy-to-use, all-round talent for difficult qPCR applications as well as for regular applications.
2X ProPlant SYBR Mix shows increased resistance against plant derived PCR inhibitors.

Applications

Quantitative PCR
Realtime PCR
Analysis of plant derived samples

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	yes
Detection	SYBR Green
Reaction Speed	Fast / Standard
Product Size	200 / 500 reactions à 20 µl
Normalization	universal ROX concentration
Sample type	DNA, cDNA
Reaction Format	2X Mastermix
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post 2X ProPlant SYBR Mix first appeared on Procomcure Biotech.