Beschreibung
1Step Magic Probe Kit is a one-step solution for the quantitative analysis of RNA. The 2X qPCR mastermix contains all components necessary for a qPCR experiment: Hotstart DNA polymerase, dNTPs (with a blend of dTTP and dUTP to allow for the digestion with UNG), and a passive reference dye, all supplied in a reaction buffer optimized for selective amplification of target sequences. For the conversion of RNA to cDNA before the amplification, VitaScript™ Reverse Transcriptase is provided as a 50X solution.
The 1Step formulation is a convenient solution as handling steps and hands-on time are reduced. Thereby, reproducibility and throughput are increased
Applications
- Quantitative PCR
- Realtime PCR
- Genexpression Analysis
- High-throughput applications
Additional information
Download the MSDS here
Download the IFU here
Specifications
| Polymerase | Taq DNA Polymerase |
| Hot Start | yes |
| Detection | probe-based |
| Reaction Speed | Fast / Standard |
| Product Size | 200 / 600 reactions à 20 µl |
| Normalization | universal ROX™ concentration |
| Sample type | RNA |
| Reaction Format | 2X Mastermix, 50X VitaScript™ separately |
| Shipping Condition | Approved for shipment on wet or dry ice |
| Storage | -15 to -25°C |
| Reverse Transcriptase | M-MuLV |
