Beschreibung
1Step Magic SYBR Kit is a one-step solution for the quantitative analysis of RNA. The 2X qPCR mastermix contains all components necessary for a qPCR experiment: Hotstart DNA polymerase, dNTPs (with a blend of dTTP and dUTP to allow for the digestion with UNG), SYBR® Green and a passive reference dye, all supplied in a reaction buffer optimized for selective amplification of target sequences. For the conversion of RNA to cDNA before the amplification, VitaScript™ Reverse Transcriptase is provided as a 50X solution.
The 1Step formulation is a convenient solution as handling steps and hands-on time are reduced. Thereby, reproducibility and throughput are increased.
Applications
- Quantitative PCR
- Realtime PCR
- Genexpression Analysis
- High-throughput applications
Additional information
Download the MSDS here
Download the IFU here
Specifications
Polymerase | Taq DNA Polymerase |
Hot Start | yes |
Detection | SYBR Green |
Reaction Speed | Fast / Standard |
Product Size | 200 / 600 reactions à 20 µl |
Normalization | universal ROX™ concentration |
Sample type | RNA |
Reaction Format | 2X Mastermix, 50X VitaScript™ separately |
Shipping Condition | Approved for shipment on wet or dry ice |
Storage | -15°C to -25°C |