Description
PhoenixDx® SARS-CoV-2 Neutralizing Antibody Test Kit (ELISA) is a competitive ELISA assay. Recombinant human ACE2 receptor protein (hACE2) was used to coat microplates, Horseradish peroxidase-labelled RBD protein (HRP-RBD) is used to prepare the enzyme conjugate.
Samples are pre-incubated with HRP-RBD outside the microplate and then transferred into the hACE2-coated plate. HRP-RBD without binding neutralizing antibodies would bind to hACE2, presence of neutralizing antibodies would block HRP-RBD from binding to hACE2. After adding a chromogenic substrate solution, the activity of HRP generates a non-ferrous product that can be quantified by measuring absorbance at 450 nm. The absorbance is inversely proportional to the effective amount of anti-RBD neutralizing antibody (nAb) in the sample. High absorbance values indicate low amounts or absence of a neutralizing antibodies.
Negative Sample
no absorbance at 450 nm
no neutralizing antibodies
Positive Sample
absorbance at 450 nm
neutralizing antibodies
Regular ELISA Assay
detects selectively only nABs detects all anti-RBD-antibodies
PhoenixDx® SARS-CoV-2 Neutralizing Antibody Test Kit regular IgG/IgM ELISA
1 Plaque Reduction Neutralization Test (PRNT) and WHO qualified calibrator validated surrogate virus neutralization test (sVNT).
The performance of PhoenixDx® SARS-CoV-2 Neutralizing Antibody Test Kit was evaluated using the WHO‘s Anti-SARS-CoV-2 Verification Panel for Serology Assays, NIBSC Code 20/B770, as at 22/09/2020. All samples of the Verification Panel were tested correctly (23 positive and 14 negative samples).
Download Manual here
Download MSDS here
Download Product Flyer here
Download Product Presentation here
