Description
Vita High Fidelity Kit provides a flexible and efficient solution for PCR applications that require a high level of fidelity. The kit provides highly efficient amplification (even for templates with up to 65% GC content) and high yields while being > 20 times more accurate than Taq Polymerase. Due to the modification to B polymerase, PCR with Vita High Fidelity Kit is not only accurate but also fast and allows for amplification of long templates up to 20 kb.
Vita High Fidelity Kit comes with 150 / 300 µl PCR-grade dNTPs (10 mM each).
Applications
- High-fidelity PCR
- Cloning and sub-cloning
- Template generation for sequencing
- High-throughput PCR
- Long PCR
- Fast PCR
- Site-directed mutagenesis
Additional information
Download the MSDS here
Download the IFU here
Specifications
| Polymerase | Engineered B-Polymerase |
| Hot Start | Built-in HotStart |
| Fidelity | > 20X Taq |
| GC-Rich PCR Performance | 65% tolerated |
| Product Overhang | blunt ends |
| Reaction Speed | Fast (15 sec / kb) |
| Product Size | 100 / 200 reactions à 50 µl |
| Endonuclease Activity | none detected |
| 5’-3’ Exonuclease Activity | low |
| 3’-5’ Proofreading Activity | yes - strong |
| Long Range PCR | up to 20 kb |
| Reaction Format | Separate Components |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Storage | -15°C to -25°C |
