Vita High Fidelity PCR Kit

95.00145.00

Vita High Fidelity PCR Kit provides a flexible and efficient solution for PCR applications that require an extra level of fidelity.

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Description

Vita High Fidelity Kit provides a flexible and efficient solution for PCR applications that require a high level of fidelity. The kit provides highly efficient amplification (even for templates with up to 65% GC content) and high yields while being > 20 times more accurate than Taq Polymerase. Due to the modification to B polymerase, PCR with Vita High Fidelity Kit is not only accurate but also fast and allows for amplification of long templates up to 20 kb.

Vita High Fidelity Kit comes with 150 / 300 µl PCR-grade dNTPs (10 mM each).

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR
  • Fast PCR
  • Site-directed mutagenesis

 

Additional information

Download the manual here
Download the MSDS here

Specifications

PolymeraseEngineered B-Polymerase
Hot StartBuilt-in HotStart
Fidelity> 20X Taq
GC-Rich PCR Performance 65% tolerated
Product Overhang blunt ends
Reaction SpeedFast (15 sec / kb)
Product Size100 / 200 reactions à 50 µl
Endonuclease Activitynone detected
5’-3’ Exonuclease Activitylow
3’-5’ Proofreading Activityyes - strong
Long Range PCRup to 20 kb
Reaction Format Separate Components
Shipping Condition Approved for shipment on Wet or Dry Ice
Storage-15°C to -25°C

Download the manual here

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