VitaProof® is a high-fidelity DNA polymerase that exhibits a 3’-5’ exonuclease activity. This feature allows the removal of falsely incorporated nucleotides making VitaProof® the ideal choice for applications that require an elevated level of fidelity.
VitaProof® PCR Kit contains VitaProof® DNA Polymerase and an optimized 10X Reaction Buffer for maximum flexibility regarding the reaction setup.
One unit of Pfu DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
|Polymerase||Pfu DNA Polymerase|
|GC-Rich PCR Performance||moderate|
|Product Overhang||blunt ends|
|Reaction Speed||Slow (1-2 min / kb)|
|Product Size||250 units / 500 units / 2500 units|
|Endonuclease Activity||none detected|
|5’-3’ Exonuclease Activity||none detected|
|3’-5’ Proofreading Activity||yes|
|Incorporation of labeled nucleotides||yes|
|Reaction Format||Separate Components|
|Shipping Condition||Approved for shipment on Wet or Dry Ice|
|Storage||-15°C to -25°C|