VitaProof® Polymerase


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VitaProof® is a high-fidelity DNA polymerase that exhibits a 3’-5’ exonuclease activity. This feature allows the removal of falsely incorporated nucleotides making VitaProof® the ideal choice for applications that require an elevated level of fidelity.

VitaProof® PCR Kit contains VitaProof® DNA Polymerase and an optimized 10X Reaction Buffer for maximum flexibility regarding the reaction setup.


  • Cloning
  • Sequencing
  • NGS

Unit definition

One unit of Pfu DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the manual here
Download the MSDS here


PolymerasePfu DNA Polymerase
Hot StartNo
Fidelity10X Taq
GC-Rich PCR Performance moderate
Product Overhang blunt ends
Reaction SpeedSlow (1-2 min / kb)
Product Size250 units / 500 units / 2500 units
Endonuclease Activitynone detected
5’-3’ Exonuclease Activitynone detected
3’-5’ Proofreading Activityyes
Incorporation of labeled nucleotidesyes
Reaction Format Separate Components
Shipping Condition Approved for shipment on Wet or Dry Ice
Storage-15°C to -25°C