Beschreibung
VitaTaq® HS DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results. The included HotStart feature allows full control over the reaction start and protects the enzyme for maximum performance.
Applications
- Routine PCR for fragments up to 5 kb
- Colony PCR
- High-throughput PCR
- Realtime PCR
- Cycle sequencing
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Additional information
Download the MSDS here
Download the IFU here
Specifications
| Polymerase | Taq DNA Polymerase |
| Hot Start | Yes |
| Fidelity | 1X Taq |
| GC-Rich PCR Performance | moderate |
| Product Overhang | 3'-A |
| Reaction Speed | Standard (1 min / kb) |
| Product Size | 200 units / 500 units / 2500 units |
| Endonuclease Activity | none detected |
| 5’-3’ Exonuclease Activity | yes |
| 3’-5’ Proofreading Activity | none detected |
| Incorporation of labeled nucleotides | yes |
| Reaction Format | Separate Components |
| Shipping Condition | Approved for shipment on wet or dry ice |
| Storage | -15°C to -25°C |
