Endpoint PCR | Procomcure Biotech

HotStart Antibody

Procomcure — Tue, 23 Jul 2019 14:00:07 +0000

HotStart Antibody is a highly pure antibody that binds to Taq DNA Polymerase and thereby blocks its polymerase activity. At the very beginning of the thermal cycling protocol, the antibody is inactivated and with this “Hot Start”, Taq DNA Polymerase regains its function. This highly controlled reaction start prevents non-specific amplification and grants maximum control over the reaction conditions.

Specifications

Target	Taq DNA Polymerase
Conjugation	Unconjugated
Concentration	5 U/µl
Storage	-15°C to -25°C

Interested in bulk sizes?

Download the IFU here

The post HotStart Antibody first appeared on Procomcure Biotech.

VitaTaq® HS PCR Kit

Procomcure — Mon, 22 Jul 2019 13:40:11 +0000

VitaTaq^® HS DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results. The included HotStart feature allows full control over the reaction start and protects the enzyme for maximum performance.

Applications

Routine PCR for fragments up to 5 kb
Colony PCR
High-throughput PCR
Realtime PCR
Cycle sequencing

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	Yes
Fidelity	1X Taq
GC-Rich PCR Performance	moderate
Product Overhang	3'-A
Reaction Speed	Standard (1 min / kb)
Product Size	200 units / 500 units / 2500 units
Endonuclease Activity	none detected
5’-3’ Exonuclease Activity	yes
3’-5’ Proofreading Activity	none detected
Incorporation of labeled nucleotides	yes
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post VitaTaq® HS PCR Kit first appeared on Procomcure Biotech.

Vita High Fidelity PCR Kit

Procomcure — Wed, 27 Mar 2019 09:25:21 +0000

Vita High Fidelity Kit provides a flexible and efficient solution for PCR applications that require a high level of fidelity. The kit provides highly efficient amplification (even for templates with up to 65% GC content) and high yields while being > 20 times more accurate than Taq Polymerase. Due to the modification to B polymerase, PCR with Vita High Fidelity Kit is not only accurate but also fast and allows for amplification of long templates up to 20 kb.

Vita High Fidelity Kit comes with 150 / 300 µl PCR-grade dNTPs (10 mM each).

Applications

High-fidelity PCR
Cloning and sub-cloning
Template generation for sequencing
High-throughput PCR
Long PCR
Fast PCR
Site-directed mutagenesis

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Engineered B-Polymerase
Hot Start	Built-in HotStart
Fidelity	> 20X Taq
GC-Rich PCR Performance	65% tolerated
Product Overhang	blunt ends
Reaction Speed	Fast (15 sec / kb)
Product Size	100 / 200 reactions à 50 µl
Endonuclease Activity	none detected
5’-3’ Exonuclease Activity	low
3’-5’ Proofreading Activity	yes - strong
Long Range PCR	up to 20 kb
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on Wet or Dry Ice
Storage	-15°C to -25°C

The post Vita High Fidelity PCR Kit first appeared on Procomcure Biotech.

VitaTaq HS Multiplex Kit

Procomcure — Thu, 29 Nov 2018 15:13:16 +0000

VitaTaq® HS Multiplex Kit is a convenient multiplex formulation of VitaTaq® DNA Polymerase optimized for applications with multiple primer pairs in one reaction. The reaction buffer propagates efficient & selective annealing of all primers to the template DNA and protects the enzyme during long PCR experiments and under harsh cycling conditions. The HotStart feature ensures full control over the amplification start and prevents non-specific amplification.

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	yes
Fidelity	1X Taq
GC-Rich PCR Performance	Low
Product Overhang	3'-A
Reaction Speed	Standard (1 min / kb)
Product Size	100 / 200 / 500 reactions à 50 µl
Final PCR Product Size	up to 2.5 kb
Multiplexing Capability	up to 20-plex
Incorporation of labeled nucleotides	yes
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post VitaTaq HS Multiplex Kit first appeared on Procomcure Biotech.

VitaProof® Polymerase

Procomcure — Fri, 16 Nov 2018 13:22:07 +0000

VitaProof® is a high-fidelity DNA polymerase that exhibits a 3’-5’ exonuclease activity. This feature allows the removal of falsely incorporated nucleotides making VitaProof® the ideal choice for applications that require an elevated level of fidelity.

VitaProof^® PCR Kit contains VitaProof^® DNA Polymerase and an optimized 10X Reaction Buffer for maximum flexibility regarding the reaction setup.

Applications

Cloning
Sequencing
NGS

Unit definition

One unit of Pfu DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Pfu DNA Polymerase
Hot Start	No
Fidelity	10X Taq
GC-Rich PCR Performance	moderate
Product Overhang	blunt ends
Reaction Speed	Slow (1-2 min / kb)
Product Size	250 units / 500 units / 2500 units
Endonuclease Activity	none detected
5’-3’ Exonuclease Activity	none detected
3’-5’ Proofreading Activity	yes
Incorporation of labeled nucleotides	yes
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on Wet or Dry Ice
Storage	-15°C to -25°C

The post VitaProof® Polymerase first appeared on Procomcure Biotech.

VitaTaq® Polymerase

Procomcure — Fri, 16 Nov 2018 09:26:33 +0000

VitaTaq^® DNA Polymerase is a thermostable DNA Polymerase that synthesizes DNA from single stranded templates. The enzyme has a 5’-3’ polymerase activity and low 5’-3’ exonuclease activity. Together with the 10X Reaction Buffer, it can work with a broad variety templates and provides robust and reliable amplification results.

VitaTaq^® PCR Kit contains VitaTaq^® DNA Polymerase and an optimized 10X Reaction Buffer for maximum flexibility regarding the reaction setup.

Applications

Routine PCR for fragments up to 5 kb
Colony PCR
High-throughput PCR
Realtime PCR
Cycle sequencing

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Additional information

Download the MSDS here

Download the IFU here

Specifications

Polymerase	Taq DNA Polymerase
Hot Start	No
Fidelity	1X Taq
GC-Rich PCR Performance	moderate
Product Overhang	3'-A
Reaction Speed	Standard (1 min / kb)
Product Size	500 units / 1000 units / 5000 units
Endonuclease Activity	none detected
5’-3’ Exonuclease Activity	yes
3’-5’ Proofreading Activity	none detected
Incorporation of labeled nucleotides	yes
Reaction Format	Separate Components
Shipping Condition	Approved for shipment on wet or dry ice
Storage	-15°C to -25°C

The post VitaTaq® Polymerase first appeared on Procomcure Biotech.